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| 25mg |
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Purity: ≥98%
Calcifediol monohydrate (also known as 25-hydroxyvitamin D3; 25-hydroxy VD3) is the major circulating metabolite of vitamin D3 in the blood and is the form that is tested in medicine to determine vitamin D deficiency, namely, the test of 25-hydroxy vitamin D (calcifediol) levels to evaluate how much vitamin D is in the body. It is also an intermediate in the biosynthetic pathway leading to the production of 1,25-dihydroxy vitamin D3, the active form of vitamin D. Calcifediol acts as a competitive inhibitor with an apparent Ki of 3.9 μM. It also suppresses PTH secretion and mRNA (ED50=2 nM). Calcifediol induced CYP24A1 expression with EC50 at 70 nM. Calcifediol stimulated the expression of thrombomodulin with EC50 at 10-100 nM. Confocal microscopy revealed that calcifediol at 0.1 - 10 μM induced VDR translocation into the nucleus dose-dependently; the VDR localization pattern was similar in cells treated with calcitriol.
| ln Vitro |
The proinflammatory cytokine KC is not affected by calcidiol or calcifediol in an ethanol solution when it comes to Pseudomonas-infected epithelial cells. Lethality of human bronchitis 16-HBE cells infected with calcifediol or calcifediol in ethanol solution was significantly reduced [1].
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| References |
[1]. Castoldi A, et al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm. 2016 Nov 22.
[2]. Wei Zheng, et al. Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β-catenin signaling. Biosci Rep. 2018 Dec 7;38(6):BSR20180595. |
| Molecular Formula |
C27H46O3
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|---|---|
| Molecular Weight |
418.66
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| Exact Mass |
418.3447
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| CAS # |
63283-36-3
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| Related CAS # |
Calcifediol;19356-17-3;Calcifediol-d6 monohydrate;2483831-70-3;Calcifediol-13C5 monohydrate
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| SMILES |
C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CCCC(C)(C)O)CC3.[H]O[H]
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| InChi Key |
WRLFSJXJGJBFJQ-WPUCQFJDSA-N
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| InChi Code |
InChI=1S/C27H44O2.H2O/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H31H2/b21-11+,22-12-/t20-,23+,24-,25+,27-/m1./s1
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| Chemical Name |
(S,Z)-3-(2-((1R,3aS,7aR,E)-1-((R)-6-hydroxy-6-methylheptan-2-yl)-7a-methyloctahydro-4H-inden-4-ylidene)ethylidene)-4-methylenecyclohexan-1-ol
hydrate
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| Synonyms |
U 32070E U-32070E U32070E 2,5-Hydroxyvitamin D3 monohydrate Calderol Dedrogyl Rayaldee
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~119.43 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.97 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3886 mL | 11.9429 mL | 23.8857 mL | |
| 5 mM | 0.4777 mL | 2.3886 mL | 4.7771 mL | |
| 10 mM | 0.2389 mL | 1.1943 mL | 2.3886 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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