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    Frequently Asked Questions

    Question 1: How stable is a compound during transportation and how should I store a compound?

    Answer: Unless specified, most products are stable at ambient temperature for a few weeks during ordinary shipping and time spent in Customs. Please store the compound at 0-4 °C for short term and -20 °C for long term use.


    Question 2: How stable is a compound in cell culture media?  Do I need to change media every day?

    Answer: Small molecule compounds are generally stable in cell culture media for at least a couple of days. In most cases, a chemical compound is freshly added to the media each time when you reconstitute or change the culture condition. In general, you do not need to change media every day unless it is clearly specified by researchers in peer-reviewed publications, or you observed a decreased effects after one day. 

     

    Question 3: Are the compounds provided by InvivoChem sensitive to freeze-thaw cycles?

    Answer: Different from proteins, peptides or other biomacromolecules, most of our small molecule compounds are not sensitive to freeze-thaw cycle unless specified. The stock solutions of a typical small molecule compound (e.g., in DMSO) can be frozen and thawed multiple times. However, we recommend you to aliquot your DMSO stock solutions (e.g. 10 mM) into 50 or 100 ul aliquots if you intend to freeze them for long term use. In some cases, a chemical compound may precipitate out at low temperature, and they have to be re-dissolved fully after warming up with tapping or vortexing of the sample tube or vial gently. 

     

    Question 4: How can I make stock solutions or formulate a compound for in vivo animal studies?

    Answer: Please send email to info@invivochem.com for any inquiries you may have regarding how to make stock solutions for in vivo animal studies. Different compounds have different solubility properties and have to be formulated differently. InvivoChem has expertise in preparing stock solutions and make formulations for in vivo animal studies, and will be happy to assist you in this regard.

     

    Question 5:  What is the stock concentration of a compound (e.g. in DMSO) do you recommend to use?

    Answer: Usually 10 mM or 20 mM. For preparing DMSO stock solutions, we suggest you to prepare a 10 mM or higher concentrations of a compound, as a more than 1000x dilution would be applied typically when making final concentrations of a compound in cell culture media, this is to minimize any solvent effects on your cells (typically less than 0.5% of DMSO might be used for most of cells).

    Typically<0.5-1% DMSO is tolerable by most cancer cells in culture media. For more sensitive cells such as primary cells, try to use <0.1% DMSO in the cell culture media. 

     

    Question 6: What should I do if I see precipitation in a DMSO solution of a compound?

    Answer: If you see a compound precipitated out in a DMSO solution, you need to re-dissolve it fully upon warming up and tapping or vortexing the sample tube or vial gently.   

    Products are for research use only;  We do not sell to patients
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