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1mg |
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2mg |
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5mg |
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25mg |
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50mg |
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Purity: ≥98%
Oligomycin A (also known as MCH 32) is a naturally occuring macrolide produced by Streptomyces. It is a potent inhibitor of ATP synthase, which blocks oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. Oligomycin A inhibits mitochondrial F0F1-ATPase with a Ki of 1 μM and shows anti-fungal activity. Oligomycin A blocks proton channel of ATP synthase, which is necessary for transforming ADP to ATP by oxidative phosphorylation, accomplishing the inhibition of ATP synthase.
ln Vitro |
Oligomycin A inhibits the mitochondrial F0F1-ATPase with a Ki of 1 μM. Oligomycin A is cytotoxic to the NCI-60 cell line, with a GI50 of 10 nM. Oligomycin A inhibits Triton X-100 solubilized ATPase with a Ki of 0.1 μM. In addition, Oligomycin A exhibits antifungal properties [1]. Oligomycin A (2.4 μM) prevents sperm from achieving viable in vitro capacitation (IVC) and inhibits progesterone-induced in vitro acrosome exocytosis (IVAE), resulting in peak O2 depletion and ATP levels [2].
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ln Vivo |
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Animal Protocol |
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References |
[1]. Salomon AR, et al. Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase. Chem Biol. 2001 Jan;8(1):71-80.
[2]. Ramió-Lluch L, et al. Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and in vitro capacitation achievement without modifying overall sperm energy levels. Reprod Fertil Dev. 2014;26(6):883-97 |
Molecular Formula |
C45H74O11
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Molecular Weight |
791.06
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CAS # |
579-13-5
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Related CAS # |
Oligomycin;1404-19-9;Oligomycin D;1404-59-7;Oligomycin C;11052-72-5;Oligomycin B;11050-94-5
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SMILES |
O1[C@@]2([H])C([H])([H])C([H])([H])[C@]([H])(C([H])=C([H])C([H])=C([H])C([H])([H])[C@@]([H])(C([H])([H])[H])[C@@]([H])([C@](C([H])([H])[H])(C([C@@]([H])(C([H])([H])[H])[C@@]([H])([C@@]([H])(C([H])([H])[H])C([C@@]([H])(C([H])([H])[H])[C@@]([H])([C@@]([H])(C([H])([H])[H])C([H])=C([H])C(=O)O[C@]([H])(C2([H])C([H])([H])[H])[C@]([H])(C([H])([H])[H])[C@]21C([H])([H])C([H])([H])[C@]([H])(C([H])([H])[H])[C@]([H])(C([H])([H])[C@@]([H])(C([H])([H])[H])O[H])O2)O[H])=O)O[H])=O)O[H])O[H])C([H])([H])C([H])([H])[H] |c:66,t:12,16|
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InChi Key |
STEWZSZUSBALCS-HQBODTQXSA-N
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InChi Code |
InChI=1S/C45H72O11/c1-12-34-17-15-13-14-16-27(4)42(51)44(11,53)43(52)32(9)40(50)31(8)39(49)30(7)38(48)26(3)18-21-37(47)54-41-29(6)35(20-19-34)55-45(33(41)10)23-22-25(2)36(56-45)24-28(5)46/h13-15,17-18,21,24-27,29-36,38,40-42,46,48,50-51,53H,12,16,19-20,22-23H2,1-11H3/b14-13+,17-15+,21-18+,28-24-/t25-,26-,27+,29+,30-,31-,32-,33-,34-,35-,36-,38+,40+,41+,42-,44+,45-/m1/s1
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Chemical Name |
(1S,2R,4E,5R,6R,6S,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,28R,29S)-22-ethyl-3,4,5,6-tetrahydro-7,11,14,15-tetrahydroxy-6-[(1Z)-2-hydroxy-1-propen-1-yl]-5,6,8,10,12,14,16,28,29-nonamethyl-spiro[2,26-dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2-[2H]pyran]-3,9,13-trione
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.2641 mL | 6.3206 mL | 12.6413 mL | |
5 mM | 0.2528 mL | 1.2641 mL | 2.5283 mL | |
10 mM | 0.1264 mL | 0.6321 mL | 1.2641 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.