Z-DEVD-FMK

Alias: ZDEVDfmk; Z-DEVD-fmk; Z DEVD fmk
Cat No.:V0029 Purity: ≥98%
Z-DEVD-FMK, atetrapeptide compound, is a novel, potent,specific, cell-permeable and irreversiblecaspase-3 inhibitorwithIC50of 18 μM.
Z-DEVD-FMK Chemical Structure CAS No.: 210344-95-9
Product category: Caspase
This product is for research use only, not for human use. We do not sell to patients.
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Z-DEVD-FMK, a tetrapeptide compound, is a novel, potent, specific, cell-permeable and irreversible caspase-3 inhibitor with IC50 of 18 μM. Compared to caspase-6, caspase-7, and caspase-10, it is relatively more selective for caspase-3.

Biological Activity I Assay Protocols (From Reference)
Targets
Caspase-3 (IC50 = 18 μM)
ln Vitro
Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner. [1] Z-DEVD-FMK significantly inhibits the activation of caspase 3 and lessens ceramide-induced cardiomyocyte death. [3] In cultured brain microvessel endothelial cells, Z-DEVD-FMK (100 μM) inhibits OxyHb's effects on DNA ladders, caspase-2 and -3 activity, cell detachment, and PARP cleavage. [4] Caspase-3 activity increases brought on by MPP+ are prevented by Z-DEVD-FMK (100 μM). With an IC50 of 18 μM, Z-DEVD-FMK inhibits 6-OHDA-induced apoptotic cell death in a dose-dependent manner. [5]
ln Vivo
Z-DEVD-FMK significantly improves neurological recovery both before and after injury by significantly reducing post-traumatic apoptosis. [2]
Enzyme Assay
Utilizing fluorescent-based substrate, the activities of caspase-3 and caspase-9 are measured. Following treatment, the cells are resuspended in 10 mM digitonin-containing lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) for 20 min at 37°C. Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 are applied to supernatants for 1 hour at 37 degrees Celsius. Fluorescence is then measured using a Gemini XS fluorescence plate reader at excitation at 400 nm and emission at 505 nm.
Cell Assay
Using the widely used MTT assay (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide), cell death in N27 cells is assessed after incubation with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK. The cells are then treated and incubated for 3 hours at 37 degrees Celsius in serum-free medium containing 0.25 mg/ml MTT. Using a SpectraMax microplate reader and a reference wavelength of 630 nm, the formation of formazan from tetrazolium is measured at 570 nm.
Animal Protocol
DMSO; 160 ng; Intracerebroventricular administration
Male Sprague Dawley rats with Brain trauma.
References

[1]. Eur J Immunol . 1998 Jan;28(1):296-304.

[2]. J Neurosci . 1997 Oct 1;17(19):7415-24.

[3]. J Card Fail . 2000 Sep;6(3):243-9.

[4]. Stroke . 2001 Feb;32(2):561-6.

[5]. Free Radic Biol Med . 2006 Nov 15;41(10):1578-89.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C30H41FN4O12
Molecular Weight
668.66
Exact Mass
668.27
Elemental Analysis
C, 53.89; H, 6.18; F, 2.84; N, 8.38; O, 28.71
CAS #
210344-95-9
Appearance
Solid powder
SMILES
CC(C)[C@@H](C(=O)N[C@@H](CC(=O)OC)C(=O)CF)NC(=O)[C@H](CCC(=O)OC)NC(=O)[C@H](CC(=O)OC)NC(=O)OCC1=CC=CC=C1
InChi Key
GBJVAVGBSGRRKN-JYEBCORGSA-N
InChi Code
InChI=1S/C30H41FN4O12/c1-17(2)26(29(42)33-20(22(36)15-31)13-24(38)45-4)35-27(40)19(11-12-23(37)44-3)32-28(41)21(14-25(39)46-5)34-30(43)47-16-18-9-7-6-8-10-18/h6-10,17,19-21,26H,11-16H2,1-5H3,(H,32,41)(H,33,42)(H,34,43)(H,35,40)/t19-,20-,21-,26-/m0/s1
Chemical Name
methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-[[(2S)-4-methoxy-4-oxo-2-(phenylmethoxycarbonylamino)butanoyl]amino]-5-oxopentanoate
Synonyms
ZDEVDfmk; Z-DEVD-fmk; Z DEVD fmk
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~100 mg/mL (~149.6 mM)
Water: <1 mg/mL (slightly soluble or insoluble)
Ethanol: <1 mg/mL
Solubility (In Vivo)
2% DMSO+30% PEG 400+5% Tween 80+ddH2O: 1mg/mL (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.4955 mL 7.4776 mL 14.9553 mL
5 mM 0.2991 mL 1.4955 mL 2.9911 mL
10 mM 0.1496 mL 0.7478 mL 1.4955 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • Z-DEVD-FMK

  • Z-DEVD-FMK

  • Z-DEVD-FMK

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